Iowa State University
College of Human Sciences and College of Agriculture College of Human Sciences College of Agriculture


Basil Nikolau

  Basil Nikolau
Professor/Biochemistry,
Biophysics & Molecular Biology

     

    Education: Ph.D., Biochemistry, Massey University, New Zealand, 1981

    Interests: Biochemical and genetic regulation of plant metabolism; plant metabolic engineering; plant genomes and functional genomics.

    Office: 2210 Molecular Biology Building
    Phone: (515) 294-9423
    Fax: (515) 294-8181

    Email: dimmas@iastate.edu

    Faculty web page (BBMB department)

Publications:


  • Fatland, B.L., Nikolau, B.J. and Wurtele, E.S. (2005) Reverse genetic characterization of cytosolic acetyl-CoA generation by ATP-Citrate lyase in Arabidopsis. Plant Cell 17, 182-203.
  • Yandeau-Nelson, M.D., Zhou, Q., Yao, H., Xu, X., Nikolau, B.J. and Schnable, P.S. (2005) MuDR transposase increases the frequency of meiotic crossovers in the vicinity of a Mu insertion in the maize a1 gene. Genetics 169, 917-929.
  • Sluszny, C., Yeung, E.S. and Nikolau, B.J. (2005) In-situ probing of the biotic-abiotic boundary of plants by laser desorption/ionization time-of-flight mass spectrometry. J. Am. Soc. Mass. Spectrom. 16, 107-115.
  • Bino, R.J., Hall, R.D., Fiehn, O., Kopka, J., Saito, K., Draper, J., Nikolau, B.J., Mendes, P., Roessner-Tunali, U., Beale, M.H., Trethewey, R.N., Lange, B.M., Wurtele, E.S. and Sumner, L.W. (2004) Potential of metabolomics as a functional genomics tool. Trends Plant Sci. 9, 418-425.
  • Nikolau, B.J., Ohlrogge, J.B. and Wurtele, E.S. (2003) Plant biotin-containing carboxylases. Arch. Biochem. Biophys. 414, 211-222.
  • Che, P., Weaver, L.M., Wurtele, E.S. and Nikolau, B.J. (2003) The role of biotin in regulating 3-methylcrotonyl-coenzyme a carboxylase expression in Arabidopsis. Plant Physiol. 131, 1479-1486.
  • Yao, H., Zhou, Q., Li, J., Smith, H., Yandeau, M., Nikolau, B.J. and Schnable, P.S. (2002) Molecular characterization of meiotic recombination across the 140-kb multigenic a1-sh2 interval of maize. Proc. Natl. Acad. Sci. USA 99, 6157-6162.
  • Xu, X., Dietrich, C.R., Lessire, R., Nikolau, B.J. and Schnable, P.S. (2002) The Endoplasmic reticulum-associated maize GL8 protein is a component of the acyl-coenzyme A elongase ivolved in the production of cuticular waxes. Plant Physiol. 128, 924-934.
  • Oliver, D.J., Nikolau, B. and Wurtele, E.S. (2002) Functional genomics: high-throughput mRNA, protein, and metabolite analyses. Metab. Eng. 4, 98-106.
  • Fatland, B.L., Ke, J., Anderson, M.D., Mentzen, W.I., Cui, L.W., Allred, C.C., Johnston, J.L., Nikolau, B.J. and Wurtele, E.S. (2002) Molecular characterization of a heteromeric ATP-citrate lyase that generates cytosolic acetyl-coenzyme A in Arabidopsis. Plant Physiol. 130, 740-756.
  • Dietrich, C.R., Cui, F., Packila, M.L., Li, J., Ashlock, D.A., Nikolau, B.J. and Schnable, P.S. (2002) Maize Mu transposons are targeted to the 5' untranslated region of the gl8 gene and sequences flanking Mu target-site duplications exhibit nonrandom nucleotide composition throughout the genome. Genetics 160, 697-716.
  • Che, P., Wurtele, E.S. and Nikolau, B.J. (2002) Metabolic and environmental regulation of 3-methylcrotonyl-coenzyme A carboxylase expression in Arabidopsis. Plant Physiol. 129, 625-637.
  • Wurtele, E.S. and Nikolau, B.J. (2000) Characterization of 3-methylcrotonyl-CoA carboxylase from plants. Methods Enzymol. 324, 280-292.
  • Nikolau, B.J., Oliver, D.J., Schnable, P.S. and Wurtele, E.S. (2000) Molecular biology of acetyl-CoA metabolism. Biochem. Soc. Trans. 28, 591-593.
  • McKean, A.L., Ke, J., Song, J., Che, P., Achenbach, S., Nikolau, B.J. and Wurtele, E.S. (2000) Molecular characterization of the non-biotin-containing subunit of 3-methylcrotonyl-CoA carboxylase. J. Biol. Chem. 275, 5582-5590.
  • Ke, J., Wen, T.N., Nikolau, B.J. and Wurtele, E.S. (2000) Coordinate regulation of the nuclear and plastidic genes coding for the subunits of the heteromeric acetyl-coenzyme A carboxylase. Plant Physiol. 122, 1057-1071.
  • Ke, J., Behal, R.H., Back, S.L., Nikolau, B.J., Wurtele, E.S. and Oliver, D.J. (2000) The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds. Plant Physiol. 123, 497-508.
  • Fatland, B., Anderson, M., Nikolau, B.J. and Wurtele, E.S. (2000) Molecular biology of cytosolic acetyl-CoA generation. Biochem. Soc. Trans. 28, 593-595.
Food Science and Human Nutrition